Author | Sampaio, Susana Carla Pires Sampaio de Oliveira | |
Author | Monteiro, Juliana Santos de Carvalho | |
Author | Cangussú, Maria Cristina Teixeira | |
Author | Santos, Gustavo Miranda Pires | |
Author | Santos, Marcos André Vannier dos | |
Author | Santos, Jean Nunes dos | |
Author | Pinheiro, Antonio Luiz Barbosa | |
Access date | 2016-07-22T14:25:46Z | |
Available date | 2016-07-22T14:25:46Z | |
Document date | 2013 | |
Citation | SAMPAIO, S. C. P. S. O. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers in Medical Sciences, v. 28, p. 799–806, 2013. | pt_BR |
ISSN | 0268-8921 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15104 | |
Language | eng | pt_BR |
Publisher | Springer Verlag | pt_BR |
Rights | open access | pt_BR |
Title | Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s10103-012-1161-9 | |
Abstract | Iron deficiency impairs the formation of hemoglobin, red blood cells, as well the transport of oxygen. The wound healing process involves numerous functions, many of which are dependent on the presence of oxygen. Laser has been shown to improve angiogenesis, increases blood supply, cell proliferation and function. We aimed to study the effect of λ660 nm laser and λ700 nm light-emitting diode (LED) on fibroblastic proliferation on cutaneous wounds on iron-deficient rodents. Induction of iron anemia was carried out by feeding 105 newborn rats with a special iron-free diet. A 1 × 1 cm wound was created on the dorsum of each animal that were randomly distributed into seven groups: I, control anemic; II, anemic no treatment; III, anemic+L; IV, anemic+LED; V, healthy no treatment; VI, healthy+laser; VII, healthy+LED (n=15 each). Phototherapy was carried out using either a diode laser (λ660 nm, 40 mW, 10 J/cm(2)) or a prototype LED device (λ700 ± 20 nm, 15 mW, 10 J/cm(2)). Treatment started immediately after surgery and was repeated at 48-h interval during 7, 14, and 21 days. After animal death, specimens were taken, routinely processed, cut, stained with hematoxylin-eosin, and underwent histological analysis and fibroblast counting. Significant difference between healthy and anemic subjects on regards the number of fibroblast between treatments was seen (p<0.008, p<0.001). On healthy animals, significant higher count was seen when laser was used (p<0.008). Anemic subjects irradiated with LED showed significantly higher count (p<0.001). It is concluded that the use of LED light caused a significant positive biomodulation of fibroblastic proliferation on anemic animals and laser was more effective on increasing proliferation on non-anemics. | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Oral Epidemiology and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Laboratório de Biologia Parasitária. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Laboratório de Biologia Parasitária. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Laboratory of Surgical Pathology. Salvador, BA, Brasil | pt_BR |
Affilliation | University of São Paulo. National Institute of Optics and Photonics. Physics Institute of São Carlos. São Paulo, SP, Brasil | pt_BR |
Subject | Anemia | pt_BR |
Subject | Phototherapy | pt_BR |
Subject | Wound healing | pt_BR |
Subject | Cell Proliferation | pt_BR |
Subject | Fibroblasts | pt_BR |
Subject | Animals | pt_BR |