Author | Oliveira, Daiana Santos de | |
Author | Figueira, Cláudio Pereira | |
Author | Zhan, L | |
Author | Pertile, Arsinoê Cristina | |
Author | Pedra, Gabriel Ghizzi | |
Author | Gusmão, I M | |
Author | Wunder Junior, Elsio Augusto | |
Author | Rodrigues, G | |
Author | Ramos, Eduardo Antônio Gonçalves | |
Author | Ko, Albert Icksang | |
Author | Childs, James E | |
Author | Reis, Mitermayer Galvão dos | |
Author | Costa, Federico | |
Access date | 2016-07-22T12:39:43Z | |
Available date | 2016-07-22T12:39:43Z | |
Document date | 2016 | |
Citation | OLIVEIRA, D. S. Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus). Epidemiology Infection, v. 144, n. 11, p. 2420-2429, 2016. | pt_BR |
ISSN | 0950-2688 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15066 | |
Language | eng | pt_BR |
Publisher | Cambridge University Press | pt_BR |
Rights | open access | pt_BR |
Title | Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus) | pt_BR |
Type | Article | pt_BR |
DOI | 10.1017/S0950268816000637 | |
Abstract | Leptospirosis is a zoonosis caused by bacteria of the genus Leptospira. The disease is globally distributed and a major public health concern. The Norway rat (Rattus norvegicus) is the main reservoir of the pathogen in urban slums of developing and developed countries. The potential routes of intra-specific leptospire transmission in rats are largely unknown. Herein, we identified pathogenic Leptospira spp. in breast tissue and milk of naturally infected rats. We examined kidney, breast tissue and milk from 24 lactating rats for the presence of leptospires using immunofluorescence, immunohistochemistry, polymerase chain reaction (PCR) and scanning electronic microscopy. All 24 rats had evidence for Leptospira in the kidneys, indicating chronic carriage. The majority of kidney-positive rats had detectable leptospires in milk (18, 75%) and breast tissue (16, 67%), as evidenced by immunofluorescence assay and immunohistochemistry. Four (17%) milk samples and two (8%) breast tissue samples were positive by quantitative real-time PCR. Scanning electron microscopy confirmed the presence of leptospires in breast tissue. No major pathological changes in breast tissue were found. This study, for the first time, identified leptospires in the milk and breast tissue of wild Norway rats, suggesting the possibility of milk-borne transmission of leptospirosis to neonates. | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA | pt_BR |
Affilliation | Ministério da Saúde. Secretaria Municipal de Saúde. Centro de Controle de Zoonoses. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA / Universidade Federal da Bahia. UFBA. Faculdade de Medicina. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, USA / Universidade Federal da Bahia. UFBA. Instituto de Saúde Coletiva. Salvador, BA, Brasil | pt_BR |
Subject | Breastfeeding | pt_BR |
Subject | Immunofluorescence | pt_BR |
Subject | Immunohistochemistry | pt_BR |
Subject | Rattus norvegicus | pt_BR |
Subject | Scanning electronic microscopy | pt_BR |
Subject | Vertical transmission | pt_BR |