Author | Martel, Nora | |
Author | Gomes, Selma A. | |
Author | Chemin, Isabelle | |
Author | Trépo, Christian | |
Author | Kay, Alan | |
Access date | 2016-07-19T11:24:57Z | |
Available date | 2016-07-19T11:24:57Z | |
Document date | 2013 | |
Citation | MARTEL, Nora: et al. Improved rolling circle amplification (RCA) of hepatitis B virus (HBV) relaxed-circular serum DNA (RC-DNA). Journal of Virological Methods, v.193, n.2, p.653-659, Nov. 2013. | pt_BR |
ISSN | 0166-0934 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14965 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Vírus da Hepatite B | pt_BR |
Subject in Portuguese | Genótipo | pt_BR |
Title | Improved rolling circle amplification (RCA) of hepatitis B virus (HBV) relaxed-circular serum DNA (RC-DNA) | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jviromet.2013.07.045 | |
Abstract | For functional analysis of HBV isolates, epidemiological studies and correct identification of recombinant genomes, the amplification of complete genomes is necessary. A method for completely in vitro amplification of full-length HBV genomes starting from serum RC-DNA is described. This uses in vitro completion/ligation of plus-strand HBV RC-DNA and amplification using Rolling-Circle Amplification, eventually followed by a genomic PCR. The method can amplify complete HBV genomes from sera with viral loads ranging from >1.0E+8 IU/ml down to 1.0E+3 IU/ml. The method can be applied to archived sera that have undergone long-term storage or to archived DNA serum extracts. The genomes can easily be cloned. HBV genotypes A-G can all be amplified with no apparent problems. A recombinant subgenotype A3/genotype E genome was identified and fully sequenced. | pt_BR |
Affilliation | Centre de Recherche en Cancérologie de Lyon (CRCL). INSERM, U1052; CNRS, UMR 5286; UCBL1, S 1052. 151 cours Albert Thomas, 69003 Lyon, France. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Centre de Recherche en Cancérologie de Lyon (CRCL). INSERM, U1052; CNRS, UMR 5286; UCBL1, S 1052. 151 cours Albert Thomas, 69003 Lyon, France. | pt_BR |
Affilliation | Centre de Recherche en Cancérologie de Lyon (CRCL). INSERM, U1052; CNRS, UMR 5286; UCBL1, S 1052. 151 cours Albert Thomas, 69003 Lyon, France / Hôpital de la Croix Rousse. Hepato-Gastroenterology Unit. Lion, France. | pt_BR |
Affilliation | Centre de Recherche en Cancérologie de Lyon (CRCL). INSERM, U1052; CNRS, UMR 5286; UCBL1, S 1052. 151 cours Albert Thomas, 69003 Lyon, France. | pt_BR |
Subject | HBV | pt_BR |
Subject | Serum relaxed-circular DNA | pt_BR |
Subject | Rolling-Circle Amplification (RCA) | pt_BR |
Subject | HBV genotypes | pt_BR |
Subject | Recombinant genomes | pt_BR |
e-ISSN | 1879-0984 | |
Embargo date | 2030-01-01 | |