| Author | Lacerda, Leandra Linhares | |
| Author | Palu, Cintia Cristina | |
| Author | Alves, Marcelo Ribeiro | |
| Author | Paredes, Bruno Diaz | |
| Author | Morrot, Alexandre | |
| Author | Silva, Maria Rosa Garcia | |
| Author | Cayota, Alfonso | |
| Author | Savino, Wilson | |
| Access date | 2016-07-07T14:43:55Z | pt_BR |
| Access date | 2016-07-08T18:49:13Z | |
| Available date | 2016-07-07T14:43:55Z | pt_BR |
| Available date | 2016-07-08T18:49:13Z | |
| Document date | 2015 | pt_BR |
| Citation | LACERDA, Leandra Linhares et al. Differential Expression of microRNAs in Thymic Epithelial Cells from Trypanosoma cruzi Acutely Infected Mice: Putative Role in Thymic Atrophy. Frontiers Immunology, v. 6, p. 1-12, Aug. 2015. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14818 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Timo | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Células epiteliais tímicas | pt_BR |
| Subject in Portuguese | Migração de Timócitos | pt_BR |
| Subject in Portuguese | MicroRNA | pt_BR |
| Title | Differential expression of microRNAs in thymic epithelial cells from Trypanosoma cruzi acutely infected mice: putative role in thymic atrophy | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fimmu.2015.00428 | pt_BR |
| Abstract | A common feature seen in acute infections is a severe atrophy of the thymus. This occurs in the murine model of acute Chagas disease. Moreover, in thymuses from Trypanosoma cruzi acutely infected mice, thymocytes exhibit an increase in the density of fibronectin and
laminin integrin-type receptors, with an increase in migratory response ex vivo. Thymic epithelial cells (TEC) play a major role in the intrathymic T cell differentiation. To date, the consequences of molecular changes promoted by parasite infection upon thymus have not been elucidated. Considering the importance of microRNA for gene expression
regulation, 85 microRNAs (mRNAs) were analyzed in TEC from T. cruzi acutely infected mice. The infection significantly modulated 29 miRNAs and modulation of 9 was also dependent whether TEC sorted out from the thymus exhibited cortical or medullary phenotype. In silico analysis revealed that these miRNAs may control target mRNAs
known to be responsible for chemotaxis, cell adhesion, and cell death. Considering that we sorted TEC in the initial phase of thymocyte loss, it is conceivable that changes in TEC miRNA expression profile are functionally related to thymic atrophy, providing new clues to better understanding the mechanisms of the thymic involution seen in experimental Chagas disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas (INI). Centro de Pesquisa Clínica em HIV/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ,Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institut Pasteur de Montevideo. Functional Genomics Unit. Montevideo, Uruguay. | pt_BR |
| Affilliation | Institut Pasteur de Montevideo. Functional Genomics Unit. Montevideo, Uruguay. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Thymus atrophy | pt_BR |
| Subject | Thymic epithelial cell | pt_BR |
| Subject | MicroRNA | pt_BR |
| Subject | Thymocyte migration | pt_BR |
