Author | Gonçalves, Verônica Dias | |
Author | Lengruber, Françoise Bohrer | |
Author | Fonseca, Bianca Oliveira | |
Author | Pereira, Renata Meirelles Santos | |
Author | Melo, Luis Dione Barbosa de | |
Author | Lopes, Ulisses Gazos | |
Author | Bello, Alexandre Ribeiro | |
Author | Pereira, José Augusto Adler | |
Access date | 2016-07-07T13:31:45Z | pt_BR |
Access date | 2016-07-08T18:49:11Z | |
Available date | 2016-07-07T13:31:45Z | pt_BR |
Available date | 2016-07-08T18:49:11Z | |
Document date | 2015 | pt_BR |
Citation | GONÇALVES, Verônica Dias; et al. Detection and characterization of multidrug-resistant enterobacteria bearing aminoglycoside-modifying gene in a university hospital at Rio de Janeiro, Brazil, along three decades. Biomédica, v.35, n.1, p.117-124, Jan./Mar. 2015. | pt_BR |
ISSN | 0120-4157 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14813 | |
Language | eng | pt_BR |
Publisher | Instituto Nacional de Salud | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Enterobacteriaceae | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | Resistência a Medicamentos | pt_BR |
Subject in Portuguese | Plasmídeos | pt_BR |
Subject in Portuguese | Acetylesterase | pt_BR |
Subject in Portuguese | Farmacorresistência Bacteriana Múltipla | pt_BR |
Title | Detection and characterization of multidrug-resistant enterobacteria bearing aminoglycoside-modifying gene in a university hospital at Rio de Janeiro, Brazil, along three decades | pt_BR |
Alternative title | Detección y caracterización de enterobacterias resistentes a múltiples fármacos, portadoras del gen modificador de aminoglucósidos en un hospital universitario de Río de Janeiro, Brasil, durante tres décadas | pt_BR |
Type | Article | pt_BR |
DOI | 10.7705/biomedica.v35i1.2276 | pt_BR |
Abstract | Introduction: Multidrug-resistant Enterobacteriaceae, particularly those resistant to gentamicin, have
become one of the most important causes of nosocomial infections.
Objective: We sought to investigate the presence of genes conferring resistance to aminoglycosides,
specially to gentamicin, in Klebsiella pneumoniae and Escherichia coli multidrug-resistant strains
isolated from different clinical materials among patients hospitalized in a university hospital in Rio de
Janeiro, Brazil.
Materials and methods: Ten colonization strains and 20 infection strains were evaluated during three
decades (1980 to 2010) using selective media containing 8 µg/ml of gentamicin. Thirty strains were
tested for antimicrobial susceptibility. Twenty two strains were subjected to plasmid DNA extraction and
12 to hybridization assays using as probe a 1.9 kb plasmid DNA fragment from one of the K. pneumoniae
strains isolated from faecal samples. This fragment was sequenced and assigned to the GQ422439
GenBank record. PCR was also performed using oligonucleotides designed for aminoglycosidemodifying
enzymes.
Results: An accC2 acetylase, besides transposons and insertion sequences, were evidenced. Twentyfour
(80%) of the isolates were positive for the aacC2 gene in agreement with antibiotic susceptibility
testing profiles, indicating the persistent presence of this gene throughout the three decades. We
detected high molecular weight plasmids in 54,5% of the strains. Of the tested strains, 91% showed
positive signal in the hybridization assays.
Conclusion: A gene codifying for one specific aminoglycoside-modifying enzyme was detected all
throughout the three decades. Our data back the adoption of preventive measures, such as a more
conscious use of antimicrobial agents in hospital environments, which can contribute to control the
dissemination of microorganisms harboring resistance gene plasmids. | pt_BR |
xmlui.metadata.dc.description.abstractes | ntroducción. Las enterobacterias resistentes a la gentamicina se asocian frecuentemente a
infecciones hospitalarias.
Objetivo. Verificar la presencia de los genes que confieren resistencia a los aminoglucósidos,
específicamente a la gentamicina, en cepas de Klebsiella pneumoniae y Escherichia coli multirresistentes,
obtenidas de pacientes internados en un hospital universitario de Río de Janeiro.
Materiales y métodos. Se recolectaron y evaluaron 10 cepas de colonización y 20 de infección entre
1980 y 2010, utilizando medios selectivos enriquecidos con gentamicina (8 µg/ml). Se obtuvieron 30 cepas en las que se determinó la resistencia a los antibióticos por medios fenotípicos. Veintidós
muestras se sometieron a extracción de ADN plasmídico y se hicieron ensayos de hibridización
en 12 de ellas, usando como sonda un fragmento de ADN plasmídico de 1,9 kb obtenido de una
cepa de K. pneumoniae aislada de muestra fecal. Este fragmento fue secuenciado y correspondió
al registro GQ422439 del GenBank. Se verificó la presencia de genes de enzimas modificadoras de
aminoglucósidos mediante reacción en cadena de la polimerasa.
Resultados. En las cepas analizadas se evidenció la presencia de la acetilasa accC2, además de
transposones y secuencias de inserción. Veinticuatro aislamientos (80 %) fueron positivos para el gen
aacC2 en concordancia con los perfiles de sensibilidad a los antibióticos, lo que indicó su persistencia
a lo largo de las tres décadas. Se detectaron plásmidos de alto peso molecular en 54,5 % de las cepas.
El 91 % de las cepas analizadas mostró signos positivos en las pruebas de hibridación.
Conclusión. Se detectó la persistencia de un gen codificador de una enzima modificadora de
aminoglucósidos a lo largo de las tres décadas. Los resultados indican que las medidas de prevención,
tales como un uso más responsable de los agentes antimicrobianos en el ambiente hospitalario,
pueden contribuir al control de la diseminación de microorganismos que albergan plásmidos de genes
de resistencia. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicase Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicase Janeiro, RJ, Brasil | pt |
Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicase Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Enterobacteriaceae | pt_BR |
Subject | Infection | pt_BR |
Subject | Drug resistance | pt_BR |
Subject | Multiple, bacterial | pt_BR |
Subject | Enzymes | pt_BR |
Subject | Aminoglycoside | pt_BR |
Subject | Plasmids | pt_BR |
Subject | Acetylesterase | pt_BR |
Subject in Spanish | Enterobacteriaceae | pt_BR |
Subject in Spanish | Infección | pt_BR |
Subject in Spanish | Farmacorresistencia bacteriana múltiple | pt_BR |
Subject in Spanish | Enzimas | pt_BR |
Subject in Spanish | Aminoglicósidos | pt_BR |
Subject in Spanish | Plásmidos | pt_BR |
Subject in Spanish | Acetilesterasa | pt_BR |