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https://www.arca.fiocruz.br/handle/icict/14625
SPECIFIC CHEMOTHERAPY OF CHAGAS DISEASE: A COMPARISON BETWEEN THE RESPONSE IN PATIENTS AND EXPERIMENTAL ANIMALS INOCULATED WITH THE SAME STRAINS
Quimioterapia
Nifurtimox
Benzonidazole
Xenodiagnóstico
Humanos
Animal
Camundongos
Brasil
Author
Affilliation
Fundação Gonçalo Moniz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Goiás. School of Medicine. Goiania, Goiania, Brasil
Fundação Gonçalo Moniz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
USP. Medical Faculty of Ribeirao Preto. Ribeirão Preto, São Paulo, SP. Brasil
Federal University of Goiás. School of Medicine. Goiania, Goiania, Brasil
Federal University of Goiás. School of Medicine. Goiania, Goiania, Brasil
Fundação Gonçalo Moniz, Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
USP. Medical Faculty of Ribeirao Preto. Ribeirão Preto, São Paulo, SP. Brasil
Federal University of Goiás. School of Medicine. Goiania, Goiania, Brasil
Abstract
Eleven strains of Ttypanosoma cruzi were isolated from patients with Chagas disease in central Brazil by
xenodiagnosis and inoculation into newborn mice. Biological characterization and isoenzyme analysis
showed that 6 strains were type II (zymodeme 2) and 5 were type III (zymodeme 1). Patients were treated
with benznidazole or benznidazole plus nifurtimox. Mice infected with each isolated strain were treated for
comparison with the results obtained in the respective patient. Evaluation of cure of the patients was based
on the indirect immunofluorescence test, complement fixation reaction and xenodiagnosis. For the mice,
haemoculture, indirect immunofluorescence testing, xenodiagnosis and inoculation of blood into newborn
mice were used. Tests were performed 3-6 months after the end of treatment. The cure rate was 66-100% in
mice infected with type II strains and O-9% in those infected with type III strains. The correlation between
treatment results in patients and mice was 8 1.8% (9 of 11 cases). Type II strains were more susceptible to
treatment, in contrast to type III strains which yielded the majority of therapeutic failures.
Keywords in Portuguese
Doença de chagasQuimioterapia
Nifurtimox
Benzonidazole
Xenodiagnóstico
Humanos
Animal
Camundongos
Brasil
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