Author | Fox, James M | |
Author | Hilburn, Silva | |
Author | Demontis, Maria-Antonietta | |
Author | Brighty, David W | |
Author | Grassi, Maria Fernanda Rios | |
Author | Castro Filho, Bernardo Galvão | |
Author | Taylor, Graham P | |
Author | Martin, Fabiola | |
Access date | 2016-05-17T12:38:02Z | |
Available date | 2016-05-17T12:38:02Z | |
Document date | 2016 | |
Citation | FOX, J. M. et al. Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo. Viruses, v. 8, p. 80, 2016. | pt_BR |
ISSN | 1999-4915 | |
ISSN | 10.3390/v8030080 | |
URI | https://www.arca.fiocruz.br/handle/icict/14305 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Title | Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo | pt_BR |
Type | Article | pt_BR |
Abstract | Clonal expansion of human T-lymphotropic virus type-1 (HTLV-1) infected cells in vivo
is well documented. Unlike human immunodeficiency virus type 1 (HIV-1), HTLV-1 plasma RNA
is sparse. The contribution of the “mitotic” spread of HTLV-1 compared with infectious spread
of the virus to HTLV-1 viral burden in established infection is uncertain. Since extrachromosomal
long terminal repeat (LTR) DNA circles are indicators of viral replication in HIV-1 carriers with
undetectable plasma HIV RNA, we hypothesised that HTLV-1 LTR circles could indicate reverse
transcriptase (RT) usage and infectious activity. 1LTR and 2LTR DNA circles were measured in
HTLV-1 cell lines and peripheral blood mononuclear cells (PBMC) of asymptomatic carriers (ACs)
and patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) or adult
T cell leukaemia/lymphoma (ATLL). 1LTR DNA circles were detected in 14/20 patients at a mean
of 1.38/100 PBMC but did not differentiate disease status nor correlate with HTLV-1 DNA copies.
2LTR DNA circles were detected in 30/31 patients and at higher concentrations in patients with
HTLV-1-associated diseases, independent of HTLV-1 DNA load. In an incident case the 2LTR DNA
circle concentration increased 2.1 fold at the onset of HAM/TSP compared to baseline. Detectable
and fluctuating levels of HTLV-1 DNA circles in patients indicate viral RT usage and virus replication.
Our results indicate HTLV-1 viral replication capacity is maintained in chronic infection and may be
associated with disease onset. | pt_BR |
Affilliation | University of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UK | pt_BR |
Affilliation | Imperial College. Section of Infectious Diseases. Department of Medicine. London, UK | pt_BR |
Affilliation | Imperial College. Section of Infectious Diseases. Department of Medicine. London, UK / The National Centre for Human Retrovirology/HTLV Clinic. Imperial College Healthcare NHS Trust St Mary’s Hospital. London NY, UK | pt_BR |
Affilliation | University of Dundee. Division of Cancer Research. Ninewells Hospital & Medical School. Dundee, UK | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | HTLV-Centre Bahian School of Medicine. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Imperial College. Section of Infectious Diseases. Department of Medicine. London, UK / The National Centre for Human Retrovirology/HTLV Clinic. Imperial College Healthcare NHS Trust St Mary’s Hospital. London NY, UK | pt_BR |
Affilliation | University of York. Centre for Immunology and Infection. Department of Biology & Hull York Medical School. York, UK | pt_BR |
Subject | Long terminal repeat | pt_BR |
Subject | LTR DNA circles | pt_BR |
Subject | Human T-lymphotropic virus | pt_BR |
Subject | HTLV | pt_BR |
Subject | Viral replication | pt_BR |