Author | Pinto, Lorena Ana | |
Author | Meira, Cássio Santana | |
Author | Villarreal, Cristiane Flora | |
Author | Santos, Marcos André Vannier dos | |
Author | Souza, Claudia V.C. de | |
Author | Ribeiro, Ivone Maria | |
Author | Tomassini, Therezinha Coelho Barbosa | |
Author | Castro Filho, Bernardo Galvão | |
Author | Soares, Milena Botelho Pereira | |
Author | Grassi, Maria Fernanda Rios | |
Access date | 2016-05-13T12:50:16Z | |
Available date | 2016-05-13T12:50:16Z | |
Document date | 2016 | |
Citation | PINTO, L. A. et al. Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy. Biomedicine and Pharmacotherapy, v. 79, p. 129-134, 2016. | pt_BR |
ISSN | 0753-3322 | |
URI | https://www.arca.fiocruz.br/handle/icict/14265 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy | pt_BR |
Type | Article | pt_BR |
DOI | dx.doi.org/10.1016/j.biopha.2016.01.041 | |
Abstract | Human T-lymphotropic virus type 1 (HTLV-1) induces a strong activation of the immune system,
especially in individuals with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. The present
study aimed to investigate the effects of physalin F on peripheral blood mononuclear cells (PBMC) of
HAM/TSP subjects. A concentration-dependent inhibition of spontaneous proliferation of PBMC from
HAM/TSP subjects was observed in the presence of physalin F, as evaluated by 3H-thymidine uptake. The
IC50 for physalin F was 0.97
0.11 mM. Flow cytometry analysis using Cytometric Bead Array (CBA)
showed that physalin F (10 mM) significantly reduced the levels of IL-2, IL-6, IL-10, TNF-a and IFN-g, but
not IL-17A, in supernatants of PBMC cultures. Next, apoptosis induction was addressed by using
flow
cytometry to evaluate annexin V expression. Treatment with physalin F (10 mM) increased the apoptotic
population of PBMC in HAM/TSP subjects. Transmission electron microscopy analysis of PBMC showed
that physalin F induced ultrastructural changes, such as pyknotic nuclei, damaged mitochondria,
enhanced autophagic vacuole formation, and the presence of myelin-like
figures. In conclusion, physalin
F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused
by HTLV-1 infection | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center of Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Subject | Physalin F | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | PBMC | pt_BR |
Subject | Immunosuppressive activity | pt_BR |
Subject | Apoptosis | pt_BR |