Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/14040
Title: Polymorphisms in IL-10 and INF-γ genes are associated with early atherosclerosis in coronary but not in carotid arteries: A study of 122 autopsy cases of young adults
Authors: Esperança, José Carlos P.
Miranda, William R. R.
B. Netto, José
Lima, Fabiane S.
Baumworcel, Leonardo
Chimelli, Leila
Silva, Rosane
Ürményi, Turán P.
Cabello, Pedro H.
Rondinelli, Edson
Faffe, Débora S.
Affilliation: Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho; Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho; Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Universidade do Grande Rio. Escola de Ciências da Saúde. Laboratório de Genética. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Clínica Médica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Metabolismo Macromolecular Firmino Torres de Castro. Rio de Janeiro, RJ, Brasil.
Abstract: Atherosclerosis is a complex disease, involving both genetic and environmental factors.However, the influence of genetic variations on its early development remains unclear. This study examined the association of 12 different polymorphismswith atherosclerosis severity in anterior descending coronary (DA, n=103) and carotid arteries (CA, n=66) of autopsied young adults (b30 years old). Histological sections (H-E) were classified according to the American Heart Association. Polymorphisms in ACE, TNF-α (−308G/A and −238 G/A), IFN-γ (+874 A/T), MMP-9 (−1562 C/T), IL-10 (−1082 A/G and −819 C/T), NOS3 (894 G/T), ApoA1 (rs964184), ApoE (E2E3E4 isoforms), and TGF-β (codons 25 and 10) genes were genotyped by gel electrophoresis or automatic DNA sequencing. Firearm projectile or car accident was the main cause of death, and no information about classical risk factors was available. Histological analysis showed high prevalence of type III atherosclerotic lesions in both DA (69%) and CA (39%) arteries, while severe type IV and V lesions were observed in 14% (DA) and 33% (CA). Allele frequencies and genotype distributions were determined. Among the polymorphisms studied, IFN-γ and IL-10 (−1082 A/G) were related to atherosclerosis severity in DA artery. No association between genotypes and lesion severity was found in CA. In conclusion, we observed that the high prevalence of early atherosclerosis in young adults is associated with IFN-γ (p b 0.001) and IL-10 (p = 0.013) genotypes. This association is blood vessel dependent. Our findings suggest that the vascular system presents site specialization, and specific genetic variations may provide future biomarkers for early disease identification.
Keywords: Atherosclerosis
IL-10
INF-y
Polymorphisms
Coronary artery disease
DeCS: Doença da Artéria Coronariana
Aterosclerose
Polimorfismo
Interleucina-10
Issue Date: 2015
Publisher: Elsevier
Citation: ESPERANÇA, José Carlos P. et al. Polymorphisms in IL-10 and INF-γ genes are associated with early atherosclerosis in coronary but not in carotid arteries: A study of 122 autopsy cases of young adults. BBA Clinical, v.3, p.214-220, 2015.
DOI: 10.1016/j.bbacli.2015.02.005
ISSN: 2214-6474
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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