Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/13752
Title: Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection.
Authors: Fukutani, Kiyoshi Ferreira
Carvalho, Cristiana Maria Costa Nascimento
Gucht, Winke Van der
Wollants, Elke
Khouri, Ricardo
Dierckx, Tim
Van Ranst, Marc
Houspie, Lieselot
Bouzas, Maiara Lana Souza Bacelar
Oliveira, Juliana R
Barral, Aldina Maria Prado
Van Weyenbergh, Johan Jozef Rosa Maria
Oliveira, Camila Indiani de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil
Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brasil
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil
Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology. School of Medicine. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Abstract: Background: Acute respiratory tract infections (ARI) present a significant morbidity and pose a global health burden. Patients are frequently treated with antibiotics although ARI are most commonly caused by virus, strengthening the need for improved diagnostic methods. Objectives: Detect viral and bacterial RNA in nasopharyngeal aspirates (NPA) from children aged 6–23 months with ARI using nCounter. Study design: A custom-designed nCounter probeset containing viral and bacterial targets was tested in NPA of ARI patients. Results: Initially, spiked control viral RNAs were detectable in ≥6.25 ng input RNA, indicating absence of inhibitors in NPA. nCounter applied to a larger NPA sample (n = 61) enabled the multiplex detection of different pathogens: RNA viruses Parainfluenza virus (PIV 1–3) and RSV A-B in 21%, Human metapneumovirus (hMPV) in 5%, Bocavirus (BoV), CoV, Influenza virus (IV) A in 3% and, Rhinovirus (RV) in 2% of samples, respectively. RSV A-B was confirmed by Real Time PCR (86.2–96.9% agreement). DNA virus (AV) was detected atRNAlevel, reflecting viral replication, in 10% of samples. Bacterial transcripts from Staphylococcus aureus, Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Mycoplasma pneumoniae and Chlamydophila pneumoniae were detected in 77, 69, 26, 8, 3 and 2% of samples, respectively. Conclusion: nCounter is robust and sensitive for the simultaneous detection of viral (both RNA and DNA) and bacterial transcripts in NPA with low RNA input (<10 ng). This medium-throughput technique will increase our understanding of ARI pathogenesis and may provide an evidence-based approach for the targeted and rational use of antibiotics in pediatric ARI.
Keywords: ARI
nCounter
Diagnostics
RSV
Issue Date: 2015
Publisher: Elsevier
Citation: FUKUTANIA, K. F. et al. Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection. Journal of Clinical Virology, v. 69, p. 190-1966, 2015.
ISSN: 1386-6532
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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