Author | Baptista, Cibele | |
Author | Miranda, Luciana de Freitas Campos | |
Author | Madeira, Maria de Fátima | |
Author | Leon, Leonor Laura Pinto | |
Author | Silva, Fátima Conceição | |
Author | Schubach, Armando de Oliveira | |
Access date | 2016-04-07T13:50:06Z | |
Available date | 2016-04-07T13:50:06Z | |
Document date | 2015 | |
Citation | BAPTISTA, Cibele; et al. In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis. Disease Markers, v.2015, Article ID 943236, 6p, 2015. | pt_BR |
ISSN | 0278-0240 | |
URI | https://www.arca.fiocruz.br/handle/icict/13638 | |
Language | eng | pt_BR |
Publisher | Hindawi Publishing Corporation | pt_BR |
Rights | open access | pt_BR |
Title | In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2015/943236 | |
Abstract | This study evaluated the in vitro sensitivity of paired Leishmania braziliensis samples isolated from the same patient before pentavalent antimonial treatment (Sample A) and after treatment failure or cutaneous leishmaniasis reactivation (Sample B) in patients undergoing intralesional administration or injections (5 mgS/kg/d) of meglumine antimoniate. Fourteen samples from 7 patients were studied. After 24 h of drug exposure, 50% lethal dose (LD50) values for promastigotes ranged from 0.37 mg/mL to 5.86 mg/mL for samples obtained before treatment (A) and 0.89 mg/mL to 7.80 mg/mL for samples obtained after treatment (B). After 48 h, LD50 values ranged from 0.37 mg/mL to 5.75 mg/mL and 0.70 mg/mL to 7.68 mg/mL for A and B samples, respectively. After 48 h, LD50 values for amastigotes ranged from 11.7 to 44.3 μg/mL for A samples and 13.7 to 52.7 μg/mL for B samples. Of 7 patients, 1 discontinued treatment and 6 were cured after retreatment with amphotericin B (4 cases) or meglumine antimoniate (2 cases). Overall the B samples had higher LD50 values than A samples; however the difference was not significant. These results do not support the hypothesis that low-dose and intralesional treatments induce selection of resistant parasites in vitro and suggest that other factors may influence therapeutic outcome in patients with poor response to initial treatment. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Núcleo de Biossegurança. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Cutaneous Leishmaniasis | pt_BR |
Subject | In vitro | pt_BR |
Subject | Leishmania (Viannia) braziliensis | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Meglumine Antimoniate Treatment | pt_BR |
DeCS | Leishmaniose cutânea | pt_BR |
DeCS | Técnicas In Vitro | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |
DeCS | Meglumina | pt_BR |
DeCS | Tratamento | pt_BR |
e-ISSN | 1875-8630 | |