Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/12963
Title: Repeated infections with Schistosoma mansoni and liver fibrosis in undernourished mice.
Authors: Coutinho, Eridan de Medeiros
Silva, Fabiana Leticia da
Barros, Andréia Ferreira de
Araújo, Roni Evencio de
Oliveira, Sheilla Andrade de
Luna, Carlos Feitosa
Barbosa Junior, Aryon de Almeida
Andrade, Zilton de Araújo
Affilliation: Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório de Imunopatolologia, Departamento de Imunologia. Recife, PE, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Abstract: The mouse model of schistosomal periportal fibrosis (Symmers’ “pipestem” fibrosis), that develops in 30–50% of the infected animals, is not reproduced in undernourished mice. Host nutritional status is likely to be a variable that may influence the outcome and progression of infection, since it interferes with the dynamics of connective tissue changes occurring in chronic hepatic schistosomiasis. Re-infections increase the occurrence of periportal liver fibrosis in well-nourished animals, but it is not known how undernourished mice would behave being repeatedly re-infected. So, 21-day-old male albino Swiss mice were individually exposed to 30 cercariae (percutaneous route) of the BH strain of Schistosoma mansoni, 4 weeks after being on a low-protein diet. Control animals were fed on a commercial balanced chow for mice. The nutritional status was evaluated by body weight gain and measurement of food intake. Mice were divided into four groups: A1 (undernourished, single infected), A2 (well-nourished, single infected), B1 (undernourished, re-infected), B2 (well-nourished, re-infected). The primary infection was performed 4 weeks after ingesting the respective diet. Re-infections started 45 days later, with exposure to 15 cercariae, at 15 day intervals. Mice were sacrificed 18 weeks after the primary exposure. The livers were submitted to morphological (gross and microscopic pathology), morphometric (percentage of fibrosis; granuloma size; volume and numerical densities) by using semi-automatic morphometry, and biochemical (quantification of collagen as hydroxyproline) studies. Worm burdens and hepatic egg counting were also recorded. Values for body weight gains were always lower in undernourished mice, the effects of re-infection being minimal on this regard. Liver and spleen weights were higher in well-nourished mice (either single infected or re-infected) and mainly related to the type of ingested diet. A greater number of re-infected well-nourished mice developed periportal fibrosis, but undernourished re-infected animals did not reproduce this lesion. The percentage of fibrosis and hepatic collagen content were higher in well-nourished mice, but differences between single infected and re-infected groups were not statistically significant
Keywords: Schistosomiasis
Liver fibrosis
Re-infections
Malnutrition
DeCS: Cirrose Hepática/parasitologia
Desnutrição Proteico-Calórica/parasitologia
Schistosoma mansoni/crescimento & desenvolvimento
Esquistossomose mansoni/parasitologia
Animais
Peso Corporal
Histocitoquímica
Hidroxiprogesteronas/metabolismo
Fígado/parasitologia
Cirrose Hepática/metabolismo
Masculino
Camundongos
Tamanho do Órgão
Contagem de Ovos de Parasitas
Desnutrição Proteico-Calórica/metabolismo
Esquistossomose mansoni/metabolismo
Issue Date: 2007
Publisher: Elsevier
Citation: COUTINHO, E. de M. Repeated infections with Schistosoma mansoni and liver fibrosis in undernourished mice. Acta Tropica, v. 101, p. 15-24, 2007.
ISSN: 0001-706X
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

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