Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/12807
Title: One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis
Authors: Pereira, Christiane Costa
Moreira, Marcela de Lima
Soares, Rodrigo Pedro Pinto
Marteleto, Bruno Henrique
Ribeiro, Vitor Marcio
Dias, Michelle Hallais França
Cardoso, Ludmila Melo
Viana, Kelvinson fernandes
Giunchetti, Rodolfo Cordeiro
Martins Filho, Olindo Assis
Araújo, Márcio Sobreira Silva
Affilliation: Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Clínica Veterinária Santo Agostinho. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro Pesquisas René Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Abstract: BACKGROUND: The main control strategy for visceral leishmaniasis in Brazil has been based on the elimination of seropositive dogs, although this is not widely accepted. In this context, the use of a long-lasting protective vaccine against canine visceral leishmaniasis (CVL) has been highly expected. The aim of this work was to determine the timeline kinetics of the cytokine microenvironment derived from circulating leukocytes as supportive immunological biomarkers triggered by Leishmune® vaccine. Cross-sectional kinetic analysis of cellular immunity cytokines was carried out at three times (1, 6 and 12 months) after primovaccination with Leishmune®. In vitro short-term whole blood cultures were stimulated with Leishmania infantum soluble antigen (SLAg). The secreted cytokine signatures and their major sources were determined. RESULTS: At six months after vaccination, Leishmune® induced an increase in IL-8, IFN-γ, IL-17a and TNF-α levels and a decrease in IL-10. Cytokine signature analysis revealed a shift in the microenvironment towards a pro-inflammatory profile mediated by IL-8 and IFN-γ. Both, CD4(+) (↑TNF-α(+) and ↑IFN-γ (+)) and CD8(+) (↑IL-17a and ↓IL-4) T-cells contributed to the acquired immune responses observed after stimulation with SLAg. CONCLUSIONS: The changes observed in the cytokine profile suggested that Leishmune® was able to induce an effective response at six months after primovaccination. After one year, it returned to baseline suggesting the need of additional boosting.
Keywords: Canine visceral leishmaniasis
Vaccine, Leishmune®
Cytokines
Issue Date: 2015
Publisher: BioMed Central
Citation: PEREIRA, Christiane Costa et al. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res., vol., 11, p. 92, 2015.
DOI: 10.1186/s12917-015-0397-6.
ISSN: 1746-6148
Copyright: open access
Appears in Collections:CPqRR - Artigos de Periódicos



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