Author | Plourde, Marie | |
Author | Ubeda, Jean-Michel | |
Author | Mandal, Goutam | |
Author | Monte Neto, Rubens Lima do | |
Author | Mukhopadhyay, Rita | |
Author | Ouellette, Marc | |
Access date | 2016-02-22T18:12:07Z | |
Available date | 2016-02-22T18:12:07Z | |
Document date | 2015 | |
Citation | PLOURDE, Marie et al. Generation of an aquaglyceroporin AQP1 null mutant in Leishmania major. Molecular and Biochemical Parasitology, v. 201, n. 2, p. 108-111, 2015. | pt_BR |
ISSN | 0166-6851 | |
URI | https://www.arca.fiocruz.br/handle/icict/12786 | |
Language | eng | pt_BR |
Publisher | Elsevier Ltda | pt_BR |
Rights | restricted access | pt_BR |
Title | Generation of an aquaglyceroporin AQP1 null mutant in Leishmania major | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.molbiopara.2015.07.003 | |
Abstract | The Leishmania aquaglyceroporin AQP1 plays an important physiological role in water and uncharged polar solutes transport, volume regulation, osmotaxis, and is a key determinant of antimony resistance. By targeted gene disruption, we generated a Leishmania major promastigote AQP1 null mutant. This required several attempts but a chromosomal null AQP1 mutant was obtained by loss of heterozygosity in the presence of a rescue plasmid encoding AQP1. Growth in the absence of selection led to the loss of the rescuing plasmid, indicating that AQP1 is not essential for Leishmania viability. The AQP1-null mutant was resistant to antimonyl tartrate (SbIII) and arsenite (AsIII) due to a decrease import of these metalloids. It also exhibited alterations in its osmoregulation abilities compared with wild-type cells. This is the first report of the generation of a genetic AQP1 null mutant in Leishmania parasite, confirming its physiological function and role in resistance to antimonials, the therapeutic mainstay against Leishmania. | pt_BR |
Affilliation | Faculté de Médecine. Université Laval. Centre de Recherche du CHU and Département de Microbiologie, Immunologie et Infectiologie. Centre de Recherche en Infectiologie. Québec, Québec, Canada. | pt_BR |
Affilliation | Faculté de Médecine. Université Laval. Centre de Recherche du CHU and Département de Microbiologie, Immunologie et Infectiologie. Centre de Recherche en Infectiologie. Québec, Québec, Canada. | pt_BR |
Affilliation | Herbert Wertheim College of Medicine. Molecular Microbiology and Infectious Diseases. Miami, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Herbert Wertheim College of Medicine. Molecular Microbiology and Infectious Diseases. Miami, USA. | pt_BR |
Affilliation | Faculté de Médecine. Université Laval. Centre de Recherche du CHU and Département de Microbiologie, Immunologie et Infectiologie. Centre de Recherche en Infectiologie. Québec, Québec, Canada. | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Aquaglyceroporin AQP1 | pt_BR |
Subject | Antimony resistance | pt_BR |
Subject | Transport assays | pt_BR |
Subject | Osmoregulation | pt_BR |