Author | Pereira, Priscilla Aparecida Tartari | |
Author | Bitencourt, Claudia da Silva | |
Author | Santos, Daiane Fernanda dos | |
Author | Nicolete, Roberto | |
Author | Gelfuso, Guilherme Martins | |
Author | Faccioli, Lúcia Helena | |
Access date | 2016-01-26T18:17:50Z | |
Document date | 2015 | |
Citation | NICOLETE, R. et al. Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions. European Journal of Pharmaceutical Sciences, p.132-139, 2015. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/12628 | |
Language | eng | pt_BR |
Publisher | ELSEVIER | pt_BR |
Rights | open access | pt_BR |
Title | Prostaglandin D2-loaded microspheres effectively activate macrophage effector functions. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.ejps.2015.07.001 | |
Abstract | Biodegradable lactic-co-glycolic acid (PLGA) microspheres (MS) improve the stability of biomolecules stability and allow enable their sustained release. Lipid mediators represent a strategy for improving host defense; however, most of these mediators, such as prostaglandin D2 (PGD2), have low water solubility and are unstable. The present study aimed to develop and characterize MS loaded with PGD2 (PGD2-MS) to obtain an innovative tool to activate macrophages. PGD2-MS were prepared using an oil-in-water emulsion solvent extraction-evaporation process, and the size, zeta potential, surface morphology and encapsulation efficiency were determined. It was also evaluated in vitro the phagocytic index, NF-jB activation, as well as nitric oxide and cytokine production by alveolar macrophages (AMs) in response to PGD2-MS. PGD2-MS were spherical with a diameter of 5.0 ± 3.3 lm and regular surface, zeta potential of ÿ13.4 ± 5.6 mV, and 36% of encapsulation efficiency, with 16–26% release of entrapped PGD2 at 4 and 48 h, respectively. PGD2-MS were more efficiently internalized by AMs than unloaded-MS, and activated NF-jB more than free PGD2. Moreover, PGD2-MS stimulated the production of nitric oxide, TNF-a, IL-1b, and TGF-b, more than free PGD2, indicating that microencapsulation increased the activating effect of PGD2 on cells. In LPS-pre-treated AMs, PGD2-MS decreased the release of IL-6 but increased the production of nitric oxide and IL-1b. These results show that the morphological characteristics of PGD2-MS facilitated interaction with, and activation of phagocytic cells; moreover, PGD2-MS retained the biological activities of PGD2 to trigger effector mechanisms in AMs. It is suggested that PGD2-MS represent a strategy for therapeutic intervention in the lungs of immunocompromised subjects. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Universidade de Brasília. Faculdade de Ciências da Saúde. Laboratório de Tecnologia de Medicamentos, Alimentos e Cosméticos. Brasília, DF, Brazil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
Subject | Microspheres | pt_BR |
Subject | PGD2 | pt_BR |
Subject | Drug delivery | pt_BR |
Subject | Phagocytosis | pt_BR |
Subject | Cytokines | pt_BR |
Subject | Alveolar macrophages | pt_BR |
Embargo date | 2016-11-30 | |