Author | Silva, Rafael Rodrigues | |
Author | Meyer, Rafael Mariante | |
Author | Silva, Andrea Alice | |
Author | Santos, Ana Luiza Barbosa dos | |
Author | Roffê, Ester | |
Author | Santiago, Helton | |
Author | Gazzinelli, Ricardo Tostes | |
Author | Vieira, Joseli Lannes | |
Access date | 2016-01-22T15:27:36Z | |
Available date | 2016-01-22T15:27:36Z | |
Document date | 2015 | |
Citation | SILVA, Rafael Rodrigues et al. Interferon-Gamma Promotes Infection of Astrocytes by Trypanosoma cruzi. PLoS One, vol. 10, n. 2, p. e0118600, 2015 | pt_BR |
ISSN | 1932-6203 | |
URI | https://www.arca.fiocruz.br/handle/icict/12594 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Interferon-Gamma Promotes Infection of Astrocytes by Trypanosoma cruzi. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0118600. eCollection 2015 | |
Abstract | The inflammatory cytokine interferon-gamma (IFNγ) is crucial for immunity against intracellular pathogens such as the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease (CD). IFNγ is a pleiotropic cytokine which regulates activation of immune and non-immune cells; however, the effect of IFNγ in the central nervous system (CNS) and astrocytes during CD is unknown. Here we show that parasite persists in the CNS of C3H/He mice chronically infected with the Colombian T. cruzi strain despite the increased expression of IFNγ mRNA. Furthermore, most of the T. cruzi-bearing cells were astrocytes located near IFNγ+ cells. Surprisingly, in vitro experiments revealed that pretreatment with IFNγ promoted the infection of astrocytes by T. cruzi increasing uptake and proliferation of intracellular forms, despite inducing increased production of nitric oxide (NO). Importantly, the effect of IFNγ on T. cruzi uptake and growth is completely blocked by the anti-tumor necrosis factor (TNF) antibody Infliximab and partially blocked by the inhibitor of nitric oxide synthesis L-NAME. These data support that IFNγ fuels astrocyte infection by T. cruzi and critically implicate IFNγ-stimulated T. cruzi-infected astrocytes as sources of TNF and NO, which may contribute to parasite persistence and CNS pathology in CD. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Departamento de Medicina Clínica. Laboratório Multidisciplinar de Apoio à Pesquisa. Rio de Janeiro, RJ, Brasil/Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, Brasil. | pt_BR |
Subject | Astrocytes/drug effects | pt_BR |
Subject | Chagas Disease/immunology | pt_BR |
Subject | Cytokines/metabolism | pt_BR |
Subject | Interferon-gamma/pharmacology | pt_BR |
Subject | NG-Nitroarginine Methyl Ester/pharmacology | pt_BR |
Subject | Trypanosoma cruzi/growth & development | pt_BR |
Subject | Interferon-gamma | pt_BR |
Subject | NG-Nitroarginine Methyl Ester | pt_BR |