Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/12569
IN VITRO SCREENING OF 5000 PLANT EXTRACTS AND IDENTIFICATION OF EICHLERIANIC ACID FROM CABRALEA CANJERANA (VELL.) MART AS ONE OF ITS TRYPANOCIDAL COMPOUND
screening
tropical plants
trypanocidal compounds
Cabralea canjerana
Meliaceae
aglaiaglabretol A.
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brasil
Abstract
Chagas' disease, caused by Trypanosoma cruzi, affects poor and neglected populations and remains a relevant public health concern in Latin America. The drug currently used in Brazil, Benznidazole, was introduced in the market 25 years ago, and is effective only in the acute phase of the disease [1]. A total of 5228 crude extracts from 1243 plant specimens from Brazil were evaluated against amastigote and tripomastigote forms of T. cruzi (Tulahuen strain expressing b-galactosidase) in L929 cell line at a single concentration of 20 mg/mL. Cell infection was done with 10 trypomastigote/cell/2 hours. Two days after the infection, samples were added and incubated for 96h. The controls used were DMSO 1% and benzonidazole at 1 µg/mL [2]. One of the active samples was the ethanol extract of Cabralea canjerana (Vell.) Mart. (Meliaceae) which presented an IC50 value of 30 mg/mL and was selected for bioassay-guided fractionation. This plant is used by indigenous people to treat inflammation of the testicles, fungal infections, meningitis and diarrhea [3, 4]. The ethanol extract of the branches was obtained by maceration and it was suspended in a mixture of ethanol:water (8:2).
Keywords
T. cruzi assayscreening
tropical plants
trypanocidal compounds
Cabralea canjerana
Meliaceae
aglaiaglabretol A.
Share