Author | Dorneles, Elaine Maria Seles | |
Author | Araújo, Márcio Sobreira Silva | |
Author | Carvalho, Andrea Teixeira | |
Author | Martins Filho, Olindo Assis | |
Author | Lage, Andrey Pereira | |
Access date | 2016-01-20T13:21:14Z | |
Available date | 2016-01-20T13:21:14Z | |
Document date | 2015 | |
Citation | DORNELES, Elaine Maria Seles et al. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants. Genet Mol Res., vol. 14, n. 1, p. 940-51, 2015 | pt_BR |
ISSN | 1676-5680 | |
URI | https://www.arca.fiocruz.br/handle/icict/12566 | |
Language | eng | pt_BR |
Publisher | FUNPEC | pt_BR |
Rights | open access | pt_BR |
Title | Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants | pt_BR |
Type | Article | pt_BR |
DOI | 10.4238/2015.February.3.1 | |
Abstract | Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-α, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested. Anti-human IL-5 mAb showed a strong cross-reactivity with cattle and goat IL-5, while anti-human TNF-α mAb showed a selective cross-reactivity with goat TNF-α. No cross-reactivity with the ruminant cytokines was observed for anti-human IL-12 and IL-13 mAbs or for the two anti-mouse cytokine mAbs tested. The present study demonstrated the cross-reactivity of various anti-human cytokine mAbs with cattle, sheep, and goat cytokines, increasing the range of immunological biomarkers for studies in veterinary medicine. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Cytokines | pt_BR |
Subject | cross-reactivity | pt_BR |
Subject | human cattle | pt_BR |
Subject | goat sheep | pt_BR |