Author | Santos Júnior, Agenor de Castro Moreira dos | |
Author | Kalume, Dário Eluan | |
Author | Camargo, Ricardo | |
Author | Gómez-Mendoza, Diana Paola | |
Author | Correa, José Raimundo | |
Author | Chameau, Sébastien | |
Author | Sousa, Marcelo Valle de | |
Author | Lima, Beatriz Dolabela de | |
Author | Ricart, Carlos André Ornelas | |
Access date | 2015-11-18T15:30:32Z | |
Available date | 2015-11-18T15:30:32Z | |
Document date | 2015 | |
Citation | SANTOS JÚNIOR, Agenor de Castro Moreira dos; et al. Unveiling the Trypanosoma cruzi Nuclear Proteome. Plos One, v.10, n.9, e0138667, 12p, Sept. 2015. | pt_BR |
ISSN | 1932-6203 | |
URI | https://www.arca.fiocruz.br/handle/icict/12272 | |
Language | eng | pt_BR |
Publisher | Plos One | pt_BR |
Rights | open access | |
Title | Unveiling the Trypanosoma cruzi Nuclear Proteome | pt_BR |
Type | Article | |
DOI | 10.1371/journal.pone.0138667 | |
Abstract | Replication of Trypanosoma cruzi, the etiological agent of Chagas disease, displays peculiar
features, such as absence of chromosome condensation and closed mitosis. Although
previous proteome and subproteome analyses of T. cruzi have been carried out, the nuclear
subproteome of this protozoan has not been described. Here, we report, for the first time to
the best of our knowledge, the isolation and proteome analysis of T. cruzi nuclear fraction.
For that, T. cruzi epimastigote cells were lysed and subjected to cell fractionation using two
steps of sucrose density gradient centrifugation. The purity of the nuclear fraction was confirmed
by phase contrast and fluorescence microscopy. Liquid chromatography coupled to
tandem mass spectrometry (LC-MS/MS) allowed the identification of 864 proteins. Among
those, 272 proteins were annotated as putative uncharacterized, and 275 had not been previously
reported on global T. cruzi proteome analysis. Additionally, to support our enrichment
method, bioinformatics analysis in DAVID was carried out. It grouped the nuclear
proteins in 65 gene clusters, wherein the clusters with the highest enrichment scores harbor
members with chromatin organization and DNA binding functions. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinas de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Affilliation | University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Chagas Disease | pt_BR |
Subject | Nucelar Proteome | pt_BR |
DeCS | Trypanosoma cruzi | pt_BR |
DeCS | Doença de Chagas | pt_BR |
DeCS | Proteoma | pt_BR |