Author | Seki, Liliane Miyuki | |
Author | Pereira, Polyana Silva | |
Author | Conceição, Magda de Souza | |
Author | Souza, Maria José | |
Author | Marques, Elizabeth Andrade | |
Author | Carballido, Jupira Miron | |
Author | Carvalho, Maria Elisabeth Serqueira de | |
Author | Assef, Ana Paula D’Alincourt Carvalho | |
Author | Asensi, Marise Dutra | |
Access date | 2015-10-02T12:45:06Z | |
Available date | 2015-10-02T12:45:06Z | |
Document date | 2013 | pt_BR |
Citation | SEKI, Liliane Miyuki; et al. Molecular epidemiology of CTX-M producing Enterobacteriaceae isolated from bloodstream infections in Rio de Janeiro, Brazil: emergence of CTX-M-15. The Brazilian Journal of Infectious Diseases, v.17, n.6, p.640-646, 2013. | pt_BR |
ISSN | 1413-8670 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/11870 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Molecular epidemiology of CTX-M producing Enterobacteriaceae isolated from bloodstream infections in Rio de Janeiro, Brazil: emergence of CTX-M-15 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.bjid.2013.03.012 | pt_BR |
Abstract | tObjective: The present study was designed to evaluate the molecular epidemiology of CTX-Mproducing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from blood-stream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil.Material and methods: A total of 231 nonduplicate Enterobacteriaceae were isolated fromfive Brazilian hospitals between September 2007 and September 2008. The antimicrobialsusceptibility testing was performed by disk diffusion method according to the Clinical Lab-oratory Standard Institute. Isolates showing resistance to third-generation cephalosporinswere screened for ESBL activity by the double-disk synergy test. The presence of blaCTX-M,blaCTX-M-15and blaKPCgenes was determined by Polymerase Chain Reaction (PCR) amplifica-tion and DNA sequencing. The molecular typing of CTX-M producing isolates was performedby pulsed-field gel electrophoresis (PFGE).Results and discussion: Ninety-three isolates were screened as ESBL positive and 85 (91%)were found to carry CTX-M-type, as follows: K. pneumoniae 59 (49%), E. cloacae 15 (42%), andE. coli 11 (15%). Ten isolates resistant for carbapenems in K. pneumoniae were blaKPC-2genepositive. Among CTX-M type isolates, CTX-M-15 was predominant in more than 50% ofisolates for K. pneumoniae, E. coli, and E. cloacae. PFGE analysis of CTX-M producing isolatesshowed the predominance of CTX-M-15 in 10 of 24 pulsotypes in K. pneumoniae, 6 of 13 in E.cloacae and 3 of 6 in E. coli. CTX-M-15 was also predominant among KPC producing isolates. In conclusion, this study showed that CTX-M-15 was circulating in Rio de Janeiro state in
2007–2008. This data reinforce the need for continuing surveillance because this scenario
may have changed over the years. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Hospital Geral de Bonsucesso. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Hospital dos Servidores do Estado. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Rio de Janeiro (UERJ). Hospital Universitário Pedro Ernesto. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Hospital Universitário Antônio Pedro. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Hospital Naval Marcílio Dias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Enterobacteriaceae | pt_BR |
Subject | Bloodstream infection | pt_BR |
Subject | CTX-M-15 | pt_BR |
Subject | PFGE | pt_BR |
Subject | KPC | pt_BR |
Subject | Brazil | pt_BR |
DeCS | Klebsiella pneumoniae | pt_BR |
DeCS | Enterobacteriaceae | pt_BR |
DeCS | Escherichia coli | pt_BR |