Author | Diemert, David J. | |
Author | Pinto, Antonio G. | |
Author | Moura, Janaina Freire de | |
Author | Jariwala, Amar | |
Author | Santiago, Helton da Costa | |
Author | Hamilton, Robert G. | |
Author | Periago, Maria Victoria | |
Author | Loukas, Alex | |
Author | Tribolet, Leon | |
Author | Mulvenna, Jason | |
Author | Oliveira, Rodrigo Correa de | |
Author | Hotez, Peter J. | |
Author | Bethony, Jeffrey Michael | |
Access date | 2015-07-28T13:11:44Z | |
Available date | 2015-07-28T13:11:44Z | |
Document date | 2012 | |
Citation | DIEMERT, David J. et al. Generalized urticaria induced by the Na-ASP-2 hookworm vaccine: implications for the development of vaccines against helminths. Journal of Allergy and Clinical Immunology, v. 130, n.1, p. 169-176, 2012 | pt_BR |
ISSN | 0091-6749 | |
ISSN | 10.1016/j.jaci.2012.04.027 | |
URI | https://www.arca.fiocruz.br/handle/icict/11413 | |
Language | eng | pt_BR |
Publisher | American Academy of Allergy | pt_BR |
Rights | restricted access | pt_BR |
Title | Generalized urticaria induced by the Na-ASP-2 hookworm vaccine: implications for the development of vaccines against helminths | pt_BR |
Type | Article | pt_BR |
Abstract | Objective: To assess the safety and immunogenicity of recombinant Na-ASP-2 adjuvanted with Alhydrogel in healthy Brazilian adults previously infected with N americanus.
Methods: Participants were randomized to receive Na-ASP-2 or hepatitis B vaccine. Major IgG and IgE epitopes of the Na-ASP-2 molecule were mapped by using sera from these same subjects. Seroepidemiologic studies in adults and children residing in hookworm-endemic areas were conducted to assess the prevalence of IgE responses to Na-ASP-2.
Results; Vaccination with a single dose of Na-ASP-2 resulted in generalized urticarial reactions in several volunteers. These reactions were associated with pre-existing Na-ASP-2–specific IgE likely induced by previous hookworm infection. Surveys revealed that a significant proportion of the population in hookworm-endemic areas had increased levels of IgE to Na-ASP-2. Epitope mapping demonstrated sites on the Na-ASP-2 molecule that are uniquely or jointly recognized by IgG and IgE antibodies.
Conclusion: Infection with N americanus induces increased levels of total and specific IgE to Na-ASP-2 that result in generalized urticaria on vaccination with recombinant Na-ASP-2. These data advance knowledge of vaccine development for helminths given their propensity to induce strong TH2 responses. Study data highlight the important differences between the immune responses to natural helminth infection and to vaccination with a recombinant helminth antigen. | pt_BR |
Affilliation | Albert B. Sabin Vaccine Institute. Washington, DC, USA/George Washington University. Department of Microbiology, Immunology and Tropical Medicine. Washington, DC, USA/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Albert B. Sabin Vaccine Institute. Washington, DC, USA | pt_BR |
Affilliation | George Washington University. Department of Microbiology, Immunology and Tropical Medicine. Washington, DC, USA/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Johns Hopkins University School of Medicine. Baltimore, MD, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | James Cook University. Queensland Tropical Health Alliance. Cairns, Australia | pt_BR |
Affilliation | James Cook University. Queensland Tropical Health Alliance. Cairns, Australia | pt_BR |
Affilliation | James Cook University. Queensland Tropical Health Alliance. Cairns, Australia/Queensland Institute of Medical Research. Brisbane, Australia | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Albert B. Sabin Vaccine Institute. Washington, DC, USA/ Baylor College of Medicine. Departments of Pediatrics and Molecular Virology and Microbiology. Houston, TX, USA | pt_BR |
Affilliation | George Washington University. Department of Microbiology, Immunology and Tropical Medicine. Washington, DC, USA/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Hookworm | pt_BR |
Subject | Necator americanus | pt_BR |
Subject | urticaria | pt_BR |
Subject | vaccine | pt_BR |
Subject | Na-ASP-2 | pt_BR |
Subject | IgE | pt_BR |