| Author | Queiroz, Rafaella Fortini Grenfell e | |
| Author | Coelho, Paulo Marcos Zech | |
| Author | Taboada, Diana | |
| Author | Mattos, Ana Carolina Alves de | |
| Author | Davis, R | |
| Author | Harn, DA | |
| Access date | 2015-07-21T18:42:23Z | |
| Available date | 2015-07-21T18:42:23Z | |
| Document date | 2014 | |
| Citation | QUEIROZ, Rafaella Fortini Grenfell et al. Newly Established Monoclonal Antibody Diagnostic Assays for Schistosoma mansoni Direct Detection in Areas of Low Endemicity. PLoS One, vol. 9, n. 1, p. e87777, 2014 | pt_BR |
| ISSN | 1932-6203 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11398 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Title | Newly Established Monoclonal Antibody Diagnostic Assays for Schistosoma mansoni Direct Detection in Areas of Low Endemicity | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0087777 | |
| Abstract | BACKGROUND: Current available methods for diagnosis of schistosomiasis mansoni lack sufficient sensitivity, which results in underreporting of infectious in areas of low endemicity.
METHODOLOGY/PRINCIPAL FINDINGS: We developed three novel diagnostic methodologies for the direct detection of schistosome infection in serum samples. These three new methods were evaluated with positive patients from a low endemicity area in southeast Brazil. The basis of the assay was the production of monoclonal antibodies against the protein backbone of heavily glycosylated Circulating Cathodic Antigen (CCA). The antibodies were also selected for having no specificity to repeating poly-Lewis x units. Assays based on the detection CCA-protein should not encounter a limitation in sensitivity due to a biological background of this particular epitope. Three diagnostic methodologies were developed and validated, (i) Immunomagnetic Separation based on improved incubation steps of non-diluted serum, (ii) Direct Enzyme-linked Immunosorbent Assay and (iii) Fluorescent Microscopy Analysis as a qualitative assay. The two quantitative assays presented high sensitivity (94% and 92%, respectively) and specificity (100%), equivalent to the analysis of 3 stool samples and 16 slides by Kato-Katz, showing promising results on the determination of cure.
CONCLUSIONS/SIGNIFICANCE: The Immunomagnetic Separation technique showed excellent correlation with parasite burden by Cohen coefficient. The qualitative method detected 47 positive individuals out of 50 with the analysis of 3 slides. This easy-to-do method was capable of discriminating positive from negative cases, even for patients with low parasite burden. | pt_BR |
| Affilliation | Fundação Oswado Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil/University of Georgia. Department of Infectious Diseases. College of Veterinary Medicine, and Center for Tropical and Emerging Global Diseases. Athens, GE, United States of America. | pt_BR |
| Affilliation | Fundação Oswadol Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswadol Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswadol Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of Georgia. College of Veterinary Medicine. Monoclonal Antibody Facility. Athens, GE, United States of America. | pt_BR |
| Affilliation | University of Georgia. Department of Infectious Diseases. College of Veterinary Medicine, and Center for Tropical and Emerging Global Diseases. Athens, GE, United States of America. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | schistosomiasis | pt_BR |
