Author | Barud, Hélida Gomes de Oliveira | |
Author | Barud, Hernane da Silva | |
Author | Cavicchioli, Maurício | |
Author | Amaral, Thais Silva do | |
Author | Oliveira Junior, Osmir Batista de | |
Author | Santos, Diego Moura | |
Author | Petersen, Antonio Luis de Oliveira Almeida | |
Author | Celes, Fabiana | |
Author | Borges, Valeria de Matos | |
Author | Oliveira, Camila Indiani de | |
Author | Oliveira, Pollyanna Francielli de | |
Author | Furtado, Ricardo Andrade | |
Author | Tavares, Denise Crispim | |
Author | Ribeiro, Sidney Jose Lima | |
Access date | 2015-07-08T13:47:12Z | |
Available date | 2015-07-08T13:47:12Z | |
Document date | 2015 | |
Citation | BARUD, H. G. O. et al. Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydrate Polymers, v. 128, p. 41-51, 2015 | pt_BR |
ISSN | 1879-1344 | |
URI | https://www.arca.fiocruz.br/handle/icict/11166 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. | pt_BR |
Type | Article | pt_BR |
DOI | 1016/j.carbpol.2015.04.07 | |
Abstract | Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites | pt_BR |
Affilliation | São Paulo State University. UNESP. School of Dentistry. Araraquara, SP, Brasil | pt_BR |
Affilliation | São Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, Brasil / Centro Universitário de Araraquara. Laboratório de Química Medicinal e Medicina Regenerativa. Araraquara, SP, Brasil | pt_BR |
Affilliation | São Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, Brasil | pt_BR |
Affilliation | São Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, Brasil | pt_BR |
Affilliation | São Paulo State University. UNESP. School of Dentistry. Araraquara, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of França. Franca, SP, Brasil | pt_BR |
Affilliation | University of França. Franca, SP, Brasil | pt_BR |
Affilliation | University of França. Franca, SP, Brasil | pt_BR |
Affilliation | São Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, Brasil | pt_BR |
Subject | Bacterial cellulose | pt_BR |
Subject | Silk fibroin | pt_BR |
Subject | Biocompatible materials | pt_BR |
Subject | Nanocomposites | pt_BR |
Subject | Scaffold | pt_BR |
Subject | Tissue engineering | pt_BR |