Author | Albuquerque, Cassiano Felippe Gonçalves de | |
Author | Burth, Patrícia | |
Author | Silva, Adriana Ribeiro | |
Author | Moraes, Isabel Matos Medeiros de | |
Author | Oliveira, Flora Magno de Jesus | |
Author | Santelli, Ricardo Erthal | |
Author | Freire, Aline Soares | |
Author | Ibrahim, Mauricio Younes | |
Author | Faria Neto, Hugo Caire de Castro | |
Author | Faria, Mauro Velho de Castro | |
Access date | 2015-05-27T13:39:50Z | |
Available date | 2015-05-27T13:39:50Z | |
Document date | 2014 | pt_BR |
Citation | ALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/10544 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Pulmão | pt_BR |
Title | Na/K-ATPase assay in the intact mice lung subjected to perfusion | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1756-0500-7-798 | pt_BR |
Abstract | Among the characteristics of acute respiratory distress syndrome (ARDS) is edema formation and its resolution depends on pneumocyte Na/K-ATPase activity. Increased concentration of oleic acid (OA) in plasma induces lung injury by targeting Na/K-ATPase and, thus, interfering in sodium transport.
FINDINGS:
Presently, we adapted a radioactivity-free assay to detect Na/K-ATPase activity in perfused lung mice, comparing the inhibitory effect of ouabain and OA. We managed to perfuse only the lung, avoiding the systemic loss of rubidium. Rb+ incorporation into lung was measured by inductively coupled plasma optical emission spectrometry (ICP OES) technique, after lung tissue digestion. Na/K-ATPase activity was the difference between Rb+ incorporation with or without ouabain. Lung Na/K-ATPase was completely inhibited by perfusion with ouabain. However, OA caused a partial inhibition.
CONCLUSIONS:
In the present work the amount of incorporated Rb+ was greater than seen in our previous report, showing that the present technique is trustworthy. This new proposed assay may allow researchers to study the importance of Na/K-ATPase activity in lung pathophysiology. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Bio-Manguinhos. Departamento de Reativos para Diagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Bio-Manguinhos. Departamento de Reativos para Diagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Lung | pt_BR |
Subject | Na/K-ATPase | pt_BR |
Subject | Inductively coupled plasma optical emission spectrometer | pt_BR |
Subject | Acute respiratory distress syndrome (ARDS) | pt_BR |
Subject | pneumocyte Na/K-ATPase | pt_BR |