Author | Santana, Marlon G. Veloso de | |
Author | Neves, Patrícia C. C. | |
Author | Santos, Juliana Ribeiro dos | |
Author | Lima, Noemia S. | |
Author | Santos, Alexandre A. C. dos | |
Author | Watkins, David I. | |
Author | Galler, Ricardo | |
Author | Ronaldo, Myrna C. | |
Access date | 2015-05-04T17:07:29Z | |
Available date | 2015-05-04T17:07:29Z | |
Document date | 2014 | pt_BR |
Citation | SANTANA, Marlon G. Veloso et al. Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment. Virology, n. 452-453, p. 202–211, 2014. | pt_BR |
ISSN | 1743-422X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/10203 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Febre Amarela | pt_BR |
Title | Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.virol.2014.01.017 | pt_BR |
Abstract | We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto deTecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Yellow Fever 17D viru | pt_BR |
Subject | Viral vector | pt_BR |
Subject | Genetic stability | pt_BR |
Subject | SIV gag IRES | pt_BR |
Subject | SUV Gag | pt_BR |
Subject | Recombinant virus | pt_BR |
Subject in Spanish | Fiebre Amarilla | pt_BR |
DeCS | Ciências Biológicas | pt_BR |