Author | Lima, Valdirene dos Santos | |
Author | Jansen, Ana Maria | |
Author | Messenger, Louisa A | |
Author | Miles, Michael A | |
Author | Llewellyn, Martin S | |
Access date | 2015-04-22T14:23:06Z | |
Available date | 2015-04-22T14:23:06Z | |
Document date | 2014 | pt_BR |
Citation | LIMA, Valdirene S. et al. Wild Trypanosoma cruzi I genetic diversity in Brazil suggests admixture and disturbance in parasite populations from the Atlantic Forest region. Parasites & Vectors, v.7, n.263, 8p, 2014. | pt_BR |
ISSN | 1756-3305 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/10091 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Parasitos | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Title | Wild Trypanosoma cruzi I genetic diversity in Brazil suggests admixture and disturbance in parasite populations from the Atlantic Forest region | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1756-3305-7-263 | pt_BR |
Abstract | Background: Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) infection is an ancient and widespread
zoonosis distributed throughout the Americas. Ecologically, Brazil comprises several distinct biomes: Amazonia,
Cerrado, Caatinga, Pantanal and the Atlantic Forest. Sylvatic T. cruzi transmission is known to occur throughout
these biomes, with multiple hosts and vectors involved. Parasite species-level genetic diversity can be a useful
marker for ecosystem health. Our aims were to: investigate sylvatic T. cruzi genetic diversity across different biomes,
detect instances of genetic exchange, and explore the possible impact of ecological disturbance on parasite
diversity at an intra-species level.
Methods: We characterised 107 isolates of T. cruzi I (TcI; discrete typing unit, DTU I) from different major Brazilian
biomes with twenty-seven nuclear microsatellite loci. A representative subset of biologically cloned isolates was
further characterised using ten mitochondrial gene loci. We compared these data generated from Brazilian TcI
isolates from around America.
Results: Genetic diversity was remarkably high, including one divergent cluster that branched outside the known
genetic diversity of TcI in the Americas. We detected evidence for mitochondrial introgression and genetic
exchange between the eastern Amazon and Caatinga. Finally, we found strong signatures of admixture among
isolates from the Atlantic Forest region by comparison to parasites from other study sites.
Conclusions: Atlantic Forest sylvatic TcI populations are highly fragmented and admixed by comparison to others
around Brazil. We speculate on: the possible causes of Atlantic Forest admixture; the role of T. cruzi as a sentinel for
ecosystem health, and the impact disrupted sylvatic transmission cycles might have on accurate source attribution
in oral outbreaks. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, UK. | pt_BR |
Affilliation | London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, UK. | pt_BR |
Affilliation | London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, UK / Bangor University. School of Biological Sciences. Molecular Ecology and Fisheries Genetics laboratory. Gwynedd, UK. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Brazil | pt_BR |
Subject | Parasites | pt_BR |
Subject in Spanish | Trypanosoma cruzi | pt_BR |
Subject in Spanish | Brasil | pt_BR |
Subject in Spanish | Parásitos | pt_BR |